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\n\n \n \n Osugi, T., Sasakura, Y., & Satake, H.\n\n\n \n \n \n \n \n The ventral peptidergic system of the adult ascidian Ciona robusta (Ciona intestinalis Type A) insights from a transgenic animal model.\n \n \n \n \n\n\n \n\n\n\n
Scientific Reports, 10(1): 1892. December 2020.\n
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@article{osugi_ventral_2020,\n\ttitle = {The ventral peptidergic system of the adult ascidian \\textit{{Ciona} robusta} (\\textit{{Ciona} intestinalis} {Type} {A}) insights from a transgenic animal model},\n\tvolume = {10},\n\tissn = {2045-2322},\n\turl = {http://www.nature.com/articles/s41598-020-58884-w},\n\tdoi = {10.1038/s41598-020-58884-w},\n\tabstract = {Abstract\n \n Ascidians are the sister group of vertebrates and occupy a critical position in explorations of the evolution of the endocrine and nervous systems of chordates. Here, we describe the complete ventral peptidergic system in adult transgenic\n Ciona robusta\n (\n Ciona intestinalis\n Type A) which expresses the\n Kaede\n reporter gene driven by the prohormone convertase 2 (PC2) gene promoter. Numerous PC2 promoter-driven fluorescent (Kaede-positive) non-neural cells were distributed in the blood sinus located at the anterior end of the pharynx, suggesting the acquisition of a peptidergic circulatory system in\n Ciona\n . Kaede-positive ciliated columnar cells, rounded cells, and tall ciliated cells were observed in the alimentary organs, including the endostyle, pharynx, esophagus, stomach, and intestine, suggesting that digestive functions are regulated by multiple peptidergic systems. In the heart, Kaede-positive neurons were located in the ring-shaped plexus at both ends of the myocardium. Nerve fiber–like tracts ran along the raphe and appeared to be connected with the plexuses. Such unique structures suggest a role for the peptidergic system in cardiac function. Collectively, the present anatomic analysis revealed the major framework of the ventral peptidergic system of adult\n Ciona\n , which could facilitate investigations of peptidergic regulation of the pharynx, endostyle, alimentary tissues, and heart.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2021-07-27},\n\tjournal = {Scientific Reports},\n\tauthor = {Osugi, Tomohiro and Sasakura, Yasunori and Satake, Honoo},\n\tmonth = dec,\n\tyear = {2020},\n\tpages = {1892},\n}\n\n\n
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\n Abstract Ascidians are the sister group of vertebrates and occupy a critical position in explorations of the evolution of the endocrine and nervous systems of chordates. Here, we describe the complete ventral peptidergic system in adult transgenic Ciona robusta ( Ciona intestinalis Type A) which expresses the Kaede reporter gene driven by the prohormone convertase 2 (PC2) gene promoter. Numerous PC2 promoter-driven fluorescent (Kaede-positive) non-neural cells were distributed in the blood sinus located at the anterior end of the pharynx, suggesting the acquisition of a peptidergic circulatory system in Ciona . Kaede-positive ciliated columnar cells, rounded cells, and tall ciliated cells were observed in the alimentary organs, including the endostyle, pharynx, esophagus, stomach, and intestine, suggesting that digestive functions are regulated by multiple peptidergic systems. In the heart, Kaede-positive neurons were located in the ring-shaped plexus at both ends of the myocardium. Nerve fiber–like tracts ran along the raphe and appeared to be connected with the plexuses. Such unique structures suggest a role for the peptidergic system in cardiac function. Collectively, the present anatomic analysis revealed the major framework of the ventral peptidergic system of adult Ciona , which could facilitate investigations of peptidergic regulation of the pharynx, endostyle, alimentary tissues, and heart.\n
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\n\n \n \n Sawada, H., Yamamoto, K., Yamaguchi, A., Yamada, L., Higuchi, A., Nukaya, H., Fukuoka, M., Sakuma, T., Yamamoto, T., Sasakura, Y., & Shirae-Kurabayashi, M.\n\n\n \n \n \n \n \n Three multi-allelic gene pairs are responsible for self-sterility in the ascidian Ciona intestinalis.\n \n \n \n \n\n\n \n\n\n\n
Scientific Reports, 10(1): 2514. December 2020.\n
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\n\n \n \n Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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@article{sawada_three_2020,\n\ttitle = {Three multi-allelic gene pairs are responsible for self-sterility in the ascidian \\textit{{Ciona} intestinalis}},\n\tvolume = {10},\n\tissn = {2045-2322},\n\turl = {http://www.nature.com/articles/s41598-020-59147-4},\n\tdoi = {10.1038/s41598-020-59147-4},\n\tabstract = {Abstract\n \n Many hermaphroditic organisms possess a self-incompatibility system to avoid inbreeding. Although the mechanisms of self-incompatibility in flowering plants are well known, little is known about the mechanisms of self-sterility in hermaphroditic marine invertebrates. Ascidians are hermaphroditic sessile marine invertebrates that release sperm and eggs into the surrounding seawater. Several species, including\n Ciona intestinalis\n type A\n (Ciona robusta)\n , exhibit strict self-sterility. In a previous study, we found that the candidate genes responsible for self-sterility in\n Ciona\n reside in chromosome 2q (locus A) and chromosome 7q (locus B). Two pairs of multi-allelic genes, named\n s(sperm)-Themis-A\n and\n v(vitelline-coat)-Themis-A\n in locus A and\n s-Themis-B\n and\n v-Themis-B\n in locus B, are responsible for self-sterility. In this study, we identified a third multi-allelic gene pair,\n s-Themis-B2\n and\n v-Themis-B2\n , within locus B that is also involved in this system. Genetic analysis revealed that the haplotypes of\n s/v-Themis-A, s/v-Themis-B\n and\n s/v-Themis-B2\n play essential roles in self-sterility. When three haplotypes were matched between\n s-Themis\n and\n v-Themis\n , fertilization never occurred even in nonself crossing. Interestingly, gene targeting of either\n s/v-Themis-B/B2\n or\n s/v-Themis-A\n by genome editing enabled self-fertilization. These results indicate that\n s/v-Themis-A, -B\n and\n -B2\n are\n S-\n determinant genes responsible for self-sterility in the ascidian\n C. intestinalis\n type A.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2021-07-27},\n\tjournal = {Scientific Reports},\n\tauthor = {Sawada, Hitoshi and Yamamoto, Kazunori and Yamaguchi, Akira and Yamada, Lixy and Higuchi, Arata and Nukaya, Haruhiko and Fukuoka, Masashi and Sakuma, Tetsushi and Yamamoto, Takashi and Sasakura, Yasunori and Shirae-Kurabayashi, Maki},\n\tmonth = dec,\n\tyear = {2020},\n\tpages = {2514},\n}\n\n\n
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\n Abstract Many hermaphroditic organisms possess a self-incompatibility system to avoid inbreeding. Although the mechanisms of self-incompatibility in flowering plants are well known, little is known about the mechanisms of self-sterility in hermaphroditic marine invertebrates. Ascidians are hermaphroditic sessile marine invertebrates that release sperm and eggs into the surrounding seawater. Several species, including Ciona intestinalis type A (Ciona robusta) , exhibit strict self-sterility. In a previous study, we found that the candidate genes responsible for self-sterility in Ciona reside in chromosome 2q (locus A) and chromosome 7q (locus B). Two pairs of multi-allelic genes, named s(sperm)-Themis-A and v(vitelline-coat)-Themis-A in locus A and s-Themis-B and v-Themis-B in locus B, are responsible for self-sterility. In this study, we identified a third multi-allelic gene pair, s-Themis-B2 and v-Themis-B2 , within locus B that is also involved in this system. Genetic analysis revealed that the haplotypes of s/v-Themis-A, s/v-Themis-B and s/v-Themis-B2 play essential roles in self-sterility. When three haplotypes were matched between s-Themis and v-Themis , fertilization never occurred even in nonself crossing. Interestingly, gene targeting of either s/v-Themis-B/B2 or s/v-Themis-A by genome editing enabled self-fertilization. These results indicate that s/v-Themis-A, -B and -B2 are S- determinant genes responsible for self-sterility in the ascidian C. intestinalis type A.\n
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\n\n \n \n Yaguchi, S., Morino, Y., & Sasakura, Y.\n\n\n \n \n \n \n \n Development of Marine Invertebrates.\n \n \n \n \n\n\n \n\n\n\n In Inaba, K., & Hall-Spencer, J. M., editor(s),
Japanese Marine Life, pages 109–124. Springer Singapore, Singapore, 2020.\n
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@incollection{inaba_development_2020,\n\taddress = {Singapore},\n\ttitle = {Development of {Marine} {Invertebrates}},\n\tisbn = {9789811513251 9789811513268},\n\turl = {http://link.springer.com/10.1007/978-981-15-1326-8_10},\n\tlanguage = {en},\n\turldate = {2022-01-25},\n\tbooktitle = {Japanese {Marine} {Life}},\n\tpublisher = {Springer Singapore},\n\tauthor = {Yaguchi, Shunsuke and Morino, Yoshiaki and Sasakura, Yasunori},\n\teditor = {Inaba, Kazuo and Hall-Spencer, Jason M.},\n\tyear = {2020},\n\tdoi = {10.1007/978-981-15-1326-8_10},\n\tpages = {109--124},\n}\n\n\n
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